Fourier transform infrared spectrometer analysis and antimicrobial screening of ethanolic extract of Operculina terpathum from cholistan desert

Pharmacy Practice

  • Ghazala Shaheen1Department of Eastern Medicine, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
  • Arslan Ashfaq2Faculty of Eastern Medicine and Natural Sciences, Ziauddin University Karachi, Pakistan.
  • Anum Khawar3Rural Health Center, RHC Chung Lahore, Pakistan.
  • Qurratul Ain Jamil4Department of Pharmacy Practice, Faculty of Pharmacy, The Islamia University of Bahawalpur, Pakistan.
  • Rashida Parveen5Superior University Lahore, Pakistan.
  • Faheem Hadi6Department of Homeopathic Medical Sciences, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
  • Aymen Owais Ghauri7Department of Eastern Medicine, Faculty of Allied Health Sciences, Jinnah University for Women, Karachi, Pakistan.
  • Jafir Hussain Shirazi8Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Pakistan.
  • Hafiz Muhammad Asif1Department of Eastern Medicine, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
  • Tahira Shamim1Department of Eastern Medicine, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
  • Laila Sumreen6Department of Homeopathic Medical Sciences, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
  • Tanveer Ali2Faculty of Eastern Medicine and Natural Sciences, Ziauddin University Karachi, Pakistan.
  • Muhammad Akram9Department of Eastern Medicine, Government College University Faisalabad, Pakistan.
  • Raessa Noor1Department of Eastern Medicine, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan.
  • Amna Mehmood2Faculty of Eastern Medicine and Natural Sciences, Ziauddin University Karachi, Pakistan.
  • Farhan Sajid10Department of Library & Information Science, Faculty of Social Sciences, The Islamia University of Bahawalpur, Pakistan.

Volume 20 Issue 2 Pages 1-6

DOI: 10.18549/PharmPract.2022.2.2647

Abstract

Aim of present study was to assess pharmacological (antioxidant, antibacterial & antifungal) potential of Operculina terpathum seeds. Ethanolic extract was prepared and its phytochemical evaluation show the different chemical compounds such as carbohydrates, phenols, tannin, flavonoids, cardiac glycosides, steroids, alkaloids and proteins. FTIR spectra showed the presence of organic acids, hydroxyl and phenolic compounds, amino groups, aliphatic compounds, functional groups such as amide, ketone, aldehyde, aromatics and halogen compounds. Antioxidant activity of the Operculina terpathum alcoholic extract was performed by DPPH method and it showed 97.13% whereas IC50±SEM (µg/ml) was 1.425±0.16. Antibacterial activity was performed against different bacterial strains and results were comparable with that of standard. Maximum antibacterial activity was exhibited by Bacillus subtillis (28.33±2 mm) and Bacillus pumilus (25.33±2 mm) respectively. Antifungal activity was also performed and it showed maximum activity against Aspergillus flavous and Candida albicans 6±1, 5±1mm respectively. These results showed that Operculina terpathum has good antibacterial and antifungal activity against different microbes and it could be used as an alternative to antibiotics, as the antibiotics resistance is very common now a days.

Keywords

  • Anti-bacterial activity
  • Anti-fungal activity
  • Agar disc diffusion method
Pharmacy Practice

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