Development of a new spectrophotometric method for the determination of flavonoids in herb of red clover (Trifolium pratense L) and zigzag clover (Trifolium medium L)
Pharmacy Practice
Alexander Vladimirovich Nikulin1I.P. Alimarin Department of Analytical Chemistry of MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies).
Fedor Valerievich Radus1I.P. Alimarin Department of Analytical Chemistry of MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies).
Ludmila Ilyinichna Fedorina1I.P. Alimarin Department of Analytical Chemistry of MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies).
Yulia Alexandrovna Efimova1I.P. Alimarin Department of Analytical Chemistry of MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies).
Mikhail Alexandrovich Lazov1I.P. Alimarin Department of Analytical Chemistry of MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies).
Elena Valeryevna Mironova1I.P. Alimarin Department of Analytical Chemistry of MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies).
Darya Aleksandrovna Trashchenkova2Pharmacology Department, First Sechenov Moscow State Medical University (Sechenov University).
Ekaterina Anatolevna Dorovskikh3Department of Pharmaceutical Natural Sciences, Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University).
Ekaterina Vyacheslavovna Sergunova3Department of Pharmaceutical Natural Sciences, Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University).
Natal'ya Vladimirovna Bobkova3Department of Pharmaceutical Natural Sciences, Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University).
Elena Nikolaevna Kostikova3Department of Pharmaceutical Natural Sciences, Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University).
Sergey Viktorovich Lutsenko4Department of Biotechnology, Sechenov First Moscow State Medical University (Sechenov University).
Valeriya Alexandrovna Savina4Department of Biotechnology, Sechenov First Moscow State Medical University (Sechenov University).
Olga Georgievna Potanina5Pharmacognosy and Industrial Pharmacy Department, Faculty of Medicine, Lomonosov Moscow State University.
Rimma Aleksandrovna Abramovich5Pharmacognosy and Industrial Pharmacy Department, Faculty of Medicine, Lomonosov Moscow State University.
Tatiana Yurievna Kovaleva3Department of Pharmaceutical Natural Sciences, Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University).
Objective: Medicinal plants containing flavonoids are attracting increasing interest of researchers due to their complex therapeutic effects on the human body. Such medicinal plants include representatives of the genus Clover - red clover (Trifolium pratense L.) and zigzag clover (Trifolium medium L.). Methods: However, spectrophotometric techniques for the determination of the total flavonoids have numerous disadvantages, which prompted the development of a new analytical technique. As a result of our research, such a technique using gallium (III) nitrate as a staining reagent instead of the traditionally used aluminium chloride was developed, and a standard for recalculation (luteolin) was substantiated. Results: It has been shown that the new methodology is capable of giving satisfactory results, is characterised by excellent metrological characteristics, meets the requirements of SP RF XV ed. and the leading pharmacopoeias of the world (EP, USP, JP). Conclusion: The proposed methodology can be recommended for use not only for scientific purposes, but also in the field of standardisation of plant raw materials at pharmaceutical industries specialising in the production of medicinal preparations of plant origin.
